Can Serum Drops Containing Doxycycline Provide a Supplemental Anti-Bacterial Effect in the Treatment of Bacterial Keratitis?

Purpose: Systemic doxycycline has been prescribed to reduce inflammation and enhance corneal healing in bacterial keratitis. Topical autologous serum drops (ASD) containing following oral supplementation may additionally confer an anti-bacterial effect. The potential of this was evaluated by determining the vitro susceptibility keratitis isolates doxycycline. Methods: minimum inhibitory concentrations (MICs) against 100 were determined using Etests. Twenty-seven Staphylococcus aureus, ten coagulase-negative Staphylococci, six Streptococcus pneumoniae, seven viridans group streptococci, other Gram-positive bacteria, nineteen Pseudomonas aeruginosa, eight Serratia marcescens, four Moraxella spp., two Haemophilus Gram-negative bacteria tested. MICs compared a standard concentration (SSCD) 4 µg/mL that would be found 50% 20% component clinical preparations ASD, corresponding SSCD (2 µg/mL) (0.8 µg/mL), respectively. equal or less than these values used deem isolate susceptible. Results: For susceptibilities SSCD, 86%, 65%, 60%, 37.2%, 23.3%, 11.6%, Chi-squared analyses comparing showed significantly greater at all three tested (<0.0001, <0.0001, <0.0001). Conclusions: Our data suggest theoretic provide additional effect treatment keratitis, especially for